Figure 1. Impaired skin barrier function following Aspergillus fumigatus patches.
(A) Experimental protocol time line. (B) One day after each patch removal, transepidermal water loss (TEWL) measurements were obtained within the patched area. (n=4 mice/group; Mean±SEM; 2-way ANOVA with Bonferroni post-tests ***p<0.001). (C) Skin inflammatory score (Kruskal-Wallis ANOVA test p<0.0001 followed by Mann-Whitney test between Aspergillus exposed WT and KO mice * p = 0.018). (D) Epidermal thickness was assessed by morphometric analysis of 3–5 pictures/mouse; representative pictures of mice taken 24h after removal of third patch (n=16–19/group; **p< 0.01 Bonferroni’s Multiple Comparison Test). (E) Plasma Asp-specific IgG1 levels are expressed as OD for a specific dilution. (n=14 mice/group; * p < 0.05 One way ANOVA with Bonferroni’s Multiple Comparison Test).
