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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: J Immunol. 2013 Aug 30;191(7):3519–3525. doi: 10.4049/jimmunol.1300789

Figure 3. TLR4 deficiency enhances IL17A skin levels after 3rd Aspergillus patch.

Figure 3

(A) IFNγ, (B) IL4, (C) IL17A and IL17F mRNA skin levels were assessed by quantitative real-time PCR after the 2nd and 3rd patch (n=5–8 and n=6–12 respectively) and expressed as a ratio over the house keeping gene HPRT (*p< 0.05 **p< 0.01 one way ANOVA with Bonferroni’s Multiple Comparison Test). (D and E) Axillary and inguinal lymph nodes cells, collected after the 3rd patch, were stimulated in vitro with Asp (30μg/ml) for 6 days. Secreted IL4 and IL17A levels were assessed by ELISA (n=5–7 mice/group; *p< 0.05 Bonferroni’s Multiple Comparison Test; n.s. not significant).