Figure 6. TLR4 and TRIF deficiencies exacerbate experimental AD.
(A) TEWL measurements after 3rd patch (n=9–13 mice/group from 2 separate experiments). (B) Skin scores were generated as described in the method section. Skin scores and (C) epidermal thickness following the third Asp-patch. (D) Plasma Asp-specific IgG1 and IgG2c levels and total IgE levels. (E) Skin mRNA levels and (F) protein levels of IL17A, IFNγ and IL4. (G) IL17F, IL6 (H) IL1β, TNFα and CXCL2 skin mRNA levels were assessed by quantitative real-time PCR after the 3rd patch (n=3–6 mice/group; representative results from 2 separate experiments; *p< 0.05, **p<0.01, one way ANOVA with Bonferroni’s Multiple Comparison Test; n.s. not significant).
