Figure 4.

Experiment setup of multidose weight curve experiments. (A) Male C57BL/6J mice were treated with compound once every 3 days. Animal weights were then monitored for 9 days. Humane end point was defined by visible signs of duress in the animals or weight loss in excess of 15% of original body weight. (B) Weight curves of compounds 1–4. (C) Histopathology analysis of sacrificed animals after multiple injections of compounds 1–4 at 1 mg/kg. + = mild damage, ++ = moderate damage, +++ = severe damage. (D) Serum levels of liver damage and kidney damage markers after three sc injections of compounds 1–3. Significantly elevated markers are shaded in gray.