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. Author manuscript; available in PMC: 2013 Oct 2.
Published in final edited form as: Osteoarthritis Cartilage. 2012 Jun 29;20(10):1197–1207. doi: 10.1016/j.joca.2012.06.002

Figure 1.

Figure 1

Intra-articular injection of hMSC promoted regeneration of rat meniscus and inhibited development of osteoarthritis after the meniscectomy. (A) Representative macroscopic findings of the meniscus 2, 4, and 8 weeks after the injection of PBS (left), hMSCs (middle), or rMSCs (right). The white dotted line indicates the regenerated tissue. Scale bar, 1 mm. (B) Area of the total meniscus injected with PBS, hMSCs, or rMSCs at 2, 4, and 8 weeks. The horizontal lines are mean values; n = 5 for each group (one-way ANOVA followed by Bonferroni post-tests. P values less than 0.017 were considered to be statistically significant.) (C) Representative sections of the meniscus stained with Toluidine Blue (top and middle), and immunostained for type II collagen (bottom) after PBS or hMSCs injection. The staining in the PBS-treated sample was less with toluidine blue and the anti-body for type II collagen. The schema of the meniscus on the left is shown for orientation. Symbols: N, native meniscus; R, regenerated meniscus; TB, Toluidine Blue; Col II, type II collagen. Scale bar, 100 μm. (D) Representative gross photographs (top) and sections (bottom) of the joint surface of the tibia at 8 weeks. The cartilage was stained with India ink to identify fibrillation and erosion. The white circle indicates the medial tibial plateau. The tibia was sectioned coronally and stained with safranin-O and fast green to identify cartilage (red). Scale bars, 2 mm (top) or 200 μm (bottom). (E) Quantification of histological analysis using the OARSI cartilage osteoarthritis histopathology grading system. Values are mean with lower and upper limit of 95 % CI; n = 5 for each group (Mann-Whitney U test). Symbols: OARSI, Osteoarthritis Research Society International.