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. Author manuscript; available in PMC: 2013 Oct 2.
Published in final edited form as: Exp Cell Res. 2011 May 18;317(14):1955–1969. doi: 10.1016/j.yexcr.2011.05.009

Figure 3. STAT3 Requires Endocytosis for Activation.

Figure 3

(A) Phosphorylation profile of STAT3 and Erk1/2 following IL-6 treatment (20 ng/mL) for the indicated period of time. Cells were treated with clathrin-mediated internalization inhibitor chlorpromazine (CPZ) or held at 4° C for 15 minutes prior to IL-6 application. Three separate replicate experiments are represented by the blots shown. (B) M67 SIE luciferase reporter activity in IL-6 treated cells that were held at 4° C, inhibited with CPZ, or treated without inhibitors for the period of time indicated. Inhibition and temperature manipulation was applied for 15 minutes prior to IL-6 treatment. Fold change is compared against cells not treated with IL-6. Neither 4 °C restricted or CPZ treated cells exhibited a significant increase in luciferase activity as compared to untreated cells either 3 or 6 hours following IL-6 treatment. Data were analyzed by two-way ANOVA and pairwise comparisons were made using the SNK method. Error bars indicate 95% confidence intervals. * indicates significantly different from untreated, p < 0.05. $ indicates significant difference between specified treatments, p < 0.05.