FIG 3.

High doses of mouse IL-4, but not IL-2, induces IL-27 resistance after just one round of priming. ELISA analysis of IL-4 produced by mouse CD4⁺ T cells that were primed with increasing doses of rmIL-4 (A) and increasing doses of rhIL-2 (B) The rest of the Th2-inducing conditions are identical to the one described for the Th2^int (see METHODS). C, IL-27 resistance was measured in CD4⁺ T cells that were primed under Th2^lo, Th2^int and Th2^hi conditions. The percentages on the top of the white bars indicate the percentage of reduction in IL-4 production. Data are representative of four (A), or two independent experiments with similar results (B, C).