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. 2013 Oct 2;8(10):e76299. doi: 10.1371/journal.pone.0076299

Figure 2. Cell death in hippocampus organotypic culture slices after KA treatment.

Figure 2

(A) Nissl staining of mature hippocampus slices displaying normal neuronal organization in both control and KA treated slices 1 week after removal of the drug. (B) Propidium Iodine (PI) staining of the cultures at 2 hours treatment with KA (KA 2h), control (Ctrl 2h) and 1 week after removal of KA (Ctrl 1 week, KA 1 week) to evaluate cell death. 12h KA (KA 12 hours) was used as a positive control for levels of cell death reported in other KA models and 15.7M KCl was used as PI staining positive control (KCl control). PI positive cells are labeled in red. White arrows point at sample PI positive cells (C) Quantification of total number of PI positive cells in the different conditions show a small but significant increase in the number of positive cells immediately after 2h KA treatment compared to control, and no significant change after 1 week recovery (n=4). * p<0.05.