Figure 4. Validation of differentially expressed milRNAs obtained in high-throughput sequencing with S-Poly(T) RT-qPCR method.

The expression levels of the selected milRNAs were assayed in both IN and CON. The 18S rRNA was used as a normalization control and the CON was served as the reference sample. All analyses were performed in biological triplicate. Statistical analysis was performed using a two-tailed Student’s t-test. Each bar represents mean ± SD. *P<0.05.