Table 3. Sensitivity of different enzyme combinations for two different samples and genes.
| Enzyme | Sample | Pol Mean c/µL (SD*) | Env Mean c/µL (SD*) |
|---|---|---|---|
| Adv2/Adv2 | 64236 Plasma | 2.0 + 0.5 | 7.5 + 2.4 |
| Phusion/Adv2 | 64236 Plasma | 8.3 + 3.4 | 4.7 + 1.5 |
| Phusion/Adv2 (25 cycles) | 64236 Plasma | 8.3 + 3.4 | 4.7 + 1.5 |
| KOD/Adv2 | 64236 Plasma | 2.1 + 0.5 | 6.0 + 2.1 |
| Phusion/Kapa HiFi | 64236 Plasma | 8.3 + 3.4 | 4.7 + 1.5 |
| Kapa HiFi/Kapa HiFi | 64236 Plasma | 6.1 + 2.1 | 4.5 + 1.7 |
| Adv2/Adv2 | pNL4-3 | 228 + 142 | 91 + 57 |
| Phusion/Adv2 | pNL4-3 | 228 + 142 | 228 + 142 |
| Phusion/Adv2 (25 cycles) | pNL4-3 | 228 + 142 | 228 + 142 |
| KOD/Adv2 | pNL4-3 | 91 + 57 | 448 + 161 |
| Phusion/Kapa HiFi | pNL4-3 | 228 + 142 | 366 + 231 |
| Kapa HiFi/Kapa HiFi | pNL4-3 | 537 + 350 | 437 + 169 |
Values are in predicted number of copies of virus per microliter of DNA derived from endpoint dilution PCR. * SD = standard deviation.