Figure 6. Double-labeling of the CNS of third instar larvae, using staining with a mouse monoclonal antibody against Drosophila synapsin (red fluorescence) and a rabbit antiserum against CCHamide-2 (green fluorescence).

A. Dorsal view of the ventral plane of the CNS, showing the central neuropil in each hemisphere and in the anterior part of the ventral nerve cord. The excitation and emission wavelengths used were selective for Alexa Fluor 594 fluorescence (red). B. The same focal plane as in A, but now using excitation and emission wavelengths selective for Alexa Fluor 488 fluorescence (green). C. Merge of A and B. The CCHamide-2 immunoreactive neuropil is located in the anterior part of the central neuropil in each hemisphere. The arrow points to a group of CCHamide-2 immunoreactive perikarya (somewhat out of focus) belonging to the group 4 perikarya of Figure 5. Scale bar = 20 µm.