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. 2013 Oct 2;8(10):e76131. doi: 10.1371/journal.pone.0076131

Figure 8. qPCR data for the expression of the D. melanogaster CCHamide-1 gene (CG14358), the CCHamide-1 receptor gene (CG14593), the CCHamide-2 gene (CG14375), and the CCHamide-2 receptor gene (CG30106), in different developmental stages.

Figure 8

Embryos, 20-24 h after egg laying (a); first instar larvae, 48 h after egg laying (b); second instar larvae, 72 h after egg laying (c); third instar larvae, 96 h after egg laying (d); pupae, 7 d after egg laying (e); and adult male (f) and female (g) flies both 10 d after egg laying, or 1 d after eclosion. At least 25 animals were pooled from each developmental stage. These pools are the same in (A)-(D). The mRNA concentrations in each panel are given relative to column g (g=1). The qPCR experiments were run as triplicates. The vertical bars in each column (which are sometimes smaller than the lines of the column) represent SEM. A. Expression of the D. melanogaster CCHamide-1 gene in the different developmental stages. B. Expression of the D. melanogaster CCHamide-1 receptor gene. C. Expression of the CCHamide-2 gene. D. Expression of the CCHamide-2 receptor gene.