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. 2013 Oct 2;8(10):e76198. doi: 10.1371/journal.pone.0076198

Figure 10. The pH dependence of repair rates of the studied adducts by PpAlkB protein.

Figure 10

Samples were assayed for extent of repair at marked pH values at 30°C. The adducts and the conditions applied for their repair were as follows: 3meC (pKa 8.7; Ueda et al. (1963) J Amer Chem 85: 4024), 100 µM Fe(II) 5 pmol PpAlkB, 5 min reaction (A) HPC (pKa 8.6; Borys-Brzywczy et al. (2005) Acta Biochim Pol 52: 149), 100 µM Fe(II) 20 pmol PpAlkB, 15 min reaction (B); HEC (pKa 5.8; Krzyzosiak et al. (1979) Polish J Chem 53: 243), 1 mM Fe(II) 20 pmol PpAlkB, 15 min reaction (C) ;εA (pKa 3.9; Secrist et al. (1972) Biochemistry 11: 3499), 3mM Fe(II) 40 pmol PpAlkB, 15 min reaction (D); and εC (pKa 3.7; Krzyzosiak et al. (1979) Polish J Chem 53: 243), 50 µM Fe(II) 80 pmol PpAlkB, 15 min reaction (E).