Table 1. Primer and probe sequences.
ID | Sequence |
---|---|
Py18S rRNA probe* | 5’ 6-FAM-CTGGCCCTTTGAGAGCCCACTGATT-BHQ-1 3’ |
Py685F* | 5’ CTTGGCTCCGCCTCGATAT 3’ |
Py782R* | 5’ TCAAAGTAACGAGAGCCCAATG 3’ |
PyCytB mRNA probe | 5’ 6-FAM-TGCACGCTACTGGTGCATCA-BHQ-1 3’ |
PyCytB Fw | 5’ GGAGTGGATGGTGTTTTAGA 3’ |
PyCytB Rv | 5’ CACCCCAATAACTCATTTGT 3’ |
Sequences from [11]. The quencher on the Py18S rRNA probe dual labelling was updated from TAMRA to BHQ-1.