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. 2013 Oct 2;8(10):e73989. doi: 10.1371/journal.pone.0073989

Figure 4. GPX activity analysis in pET-28a, pET-W69 and pET-W106.

Figure 4

(A) SDS-PAGE analysis of the expression of recombinant proteins in E. coli. The complete protein of the bacteria and purified recombinant protein from soluble crude extract were separated by 12% SDS-PAGE and stained with Coomassie Brilliant Blue. M, protein size marker (16–94 kDa). T, total proteins of the bacteria (10 μg); P, purified recombinant proteins (1 μg) from soluble crude extract. (B) Enzyme activities of the GPX isoenzymes were calculated with H2O2 and t-BHP as the substrates. Data are means ±SD of three independent experiments.