Table 1. Strains, plasmids and primers used in this study.
| Bacterial strain, plasmid or primer names | Relevant characteristic or sequence | Source or enzyme site |
|---|---|---|
| Strains | ||
| B. amyloliquefaciens | ||
| B10-127 | Laboratory stock | |
| pBDH | B10-127 with pMA5-bdh | This study |
| pGAP | B10-127 with pMA5-gapA | This study |
| pBG | B10-127 with pMA5-bdh-HapII-gapA | This study |
| E. coli JM109 | Laboratory stock | |
| Plasmids | ||
| pMA5-HapII | Expression vector (in E. Coli, Apr; in B. amyloliquefaciens, Kanr) | Laboratory stock |
| pMA5-bdh | pMA5-HapII with bdh (in E. Coli, Apr; in B. amyloliquefaciens, Kanr) | This study |
| pMA5-gapA | pMA5-HapII with gapA (in E. Coli, Apr; in B. amyloliquefaciens, Kanr) | This study |
| pMA5-bdh- HapII-gapA | Apr, Kanr; pMA5-HapII with bdh and HapII-gapA (in E. Coli, Apr; in B. amyloliquefaciens, Kanr) | This study |
| Primers | ||
| P1 | 5′-CATATGATGAAAGCGGCAAGATGGC-3′ | NdeI |
| P2 | 5′-GGATCCTTAATTCGGTTTTACTAAG-3′ | BamHI |
| P3 | 5′-GGATCCATGGCAGTAAAAGTCGG-3′ | BamHI |
| P4 | 5′-ACGCGTTTAAAGACCTTGTTTTGCG-3′ | MluI |
| P5 | 5′-ACGCGT TTTTGAGTGATCTTCTC-3′ | MluI |
Underlined nucleotides are the restriction enzyme sites