Table 1. Strains, plasmids and primers used in this study.
Bacterial strain, plasmid or primer names | Relevant characteristic or sequence | Source or enzyme site |
---|---|---|
Strains | ||
B. amyloliquefaciens | ||
B10-127 | Laboratory stock | |
pBDH | B10-127 with pMA5-bdh | This study |
pGAP | B10-127 with pMA5-gapA | This study |
pBG | B10-127 with pMA5-bdh-HapII-gapA | This study |
E. coli JM109 | Laboratory stock | |
Plasmids | ||
pMA5-HapII | Expression vector (in E. Coli, Apr; in B. amyloliquefaciens, Kanr) | Laboratory stock |
pMA5-bdh | pMA5-HapII with bdh (in E. Coli, Apr; in B. amyloliquefaciens, Kanr) | This study |
pMA5-gapA | pMA5-HapII with gapA (in E. Coli, Apr; in B. amyloliquefaciens, Kanr) | This study |
pMA5-bdh- HapII-gapA | Apr, Kanr; pMA5-HapII with bdh and HapII-gapA (in E. Coli, Apr; in B. amyloliquefaciens, Kanr) | This study |
Primers | ||
P1 | 5′-CATATGATGAAAGCGGCAAGATGGC-3′ | NdeI |
P2 | 5′-GGATCCTTAATTCGGTTTTACTAAG-3′ | BamHI |
P3 | 5′-GGATCCATGGCAGTAAAAGTCGG-3′ | BamHI |
P4 | 5′-ACGCGTTTAAAGACCTTGTTTTGCG-3′ | MluI |
P5 | 5′-ACGCGT TTTTGAGTGATCTTCTC-3′ | MluI |
Underlined nucleotides are the restriction enzyme sites