A representative data set showing multiparametric MRI-derived maps obtained before (I1t) and after reperfusion (R1t) from an animal subjected to 3 h of transient focal ischemia. Top (left to right): cerebral blood flow (CBF), apparent diffusion coefficient (ADCw) and transverse relaxation time (T2) maps. Middle (left to right): spin–lattice relaxation times in the absence and presence of off-resonance saturation (T1 and T1sat, respectively), the forward rate of magnetization transfer (Kfor), and magnetization transfer ratio (MTR) maps. Bottom row: A corresponding ISODATA segmentation theme map to demonstrate the ROIs that were selected to represent ischemic tissue with (red and green) and without (yellow) BBB disruption (left). To the right, are a corresponding 14C-AIB autoradiographic image and a cresyl violet stained histologic section that were used to confirm acute BBB damage and the region of ischemic damage, respectively. (From Knight et al. Magn Reson Med. 2005;54:822-832)