PCMT1 overexpression Inhibits Mst1 induced cardiomyocyte apoptosis. A, NRCMs were transduced with either Ad-LacZ or Ad-PCMT1 (MOI=50). Forty-eight hours after transduction, PCMT1 expression was analyzed by Western blot analysis. B, NRCMs were transduced with Ad-LacZ, Ad-Mst1, or Ad-PCMT1 with different combinations (total MOI=100). Forty-eight hours after transduction, cell apoptosis was quantitated by Annexin-V (AV)–fluorescein isothiocyanate (FITC) stain detection kit (BioVision). C, Histone-associated DNA fragments were quantitated by the Cell Death Detection ELISA. The data are representatives of 4 independent experiments.