Figure 3.

AsSn transfection inhibits cell motility in vitro. (A) Wound-healing ability of AsSn cells vs. mock-transfected cells. Mock-transfected cells frequently migrated into the wound area and filled in the wound after 24 h, whereas the AsSn monolayer migrated slowly into the wound. (B) Cell migration was estimated by means of Transwell migration assays. The numbers of migrated cells were estimated 10 h after seeding, then migrated cells were fixed, stained and photographed. The experiment was repeated three times. The invasion ability of the AsSn cells was significantly lower than that of the mock-transfected cells (P<0.05 for both). (C) Growth curves showing that there was no difference between the mock-transfected and AsSn cells in cell growth until 72 h after seeding. AsSn, antisense-Snail cDNA construct.