Figure 3.

ROS mediates cell death induced by TMPZ. SGC7901 cells were treated with the indicated concentration of TMPZ in the absence or presence of 5 mM NAC for 24 h. (A) TMPZ induces dose-dependent ROS accumulation. SGC7901 cells were treated with 4, 6 or 8 mM TMPZ for 24 h. Cells were stained with DCFH-DA and analyzed by flow cytometry. (B) Quantitative analysis of the percentage of ROS production determined by FACS analysis. Data are expressed as the mean fluorescence intensity and represent the mean ± SD of three independent experiments. ^**P<0.01. (C) ROS mediate the reduction of cell viability induced by TMPZ. Cell viability was analyzed by the MTT method. The values represent the mean ± SD of three independent experiments. ^**P<0.01. ROS, reactive oxygen species; TMPZ, tetramethylpyrazine; NAC, N-acetylcysteine; DCFH-DA, 2’7’-dichlorodihydrofluorescein diacetate; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide.