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Association between the expression of TIM-3 and certain bio-markers of EMT in osteosarcoma sections detected by immunofluorescent dual staining. Dual immunofluorescence staining showed that the expression of certain biomarkers of EMT, including Slug, Snail and Smad, were co-expressed with TIM-3 in the same carcinoma cells. Nuclei were stained with DAPI. Scale bar, 20 μm. EMT, epithelial-mesenchymal transition. TIM, T cell Ig- and mucin-domain-containing molecule.
