Table I.
Immunohistochemical study: Antibodies, source and dilution.
| Ab | Dilution | Clone | Source |
|---|---|---|---|
| TIM-1 | 1:100 | Polyclonal Goat IgG | R&D System |
| TIM-3 | 1:100 | Polyclonal Goat IgG | R&D System |
| TIM-4 | 1:100 | Polyclonal Goat IgG | R&D System |
| CD3 | 1:50 | Monoclonal mouse IgG (F7.2.38) | Dako |
| CK-18 | 1:400 | Monoclonal mouse IgG (C-04) | Santa Cruz |
| CD68 | 1:50 | Monoclonal mouse IgG (3F103) | Santa Cruz |
| CD31 | 1:50 | Monoclonal mouse IgG (10G9) | Santa Cruz |
| CD1a | 1:200 | Monoclonal mouse IgG (7A7) | Abcam |
| PCNA | 1:200 | Monoclonal mouse IgG (F-2) | Santa Cruz |
| Bcl-2 | 1:100 | Monoclonal mouse IgG (C-2) | Santa Cruz |
| Slug | 1:100 | Monoclonal mouse IgG | Abcam |
| Snail | 1:100 | Polyclonal Goat IgG | Abcam |
| Smad2 | 1:100 | Polyclonal Goat IgG | Santa Cruz |
| Smad3 | 1:100 | Polyclonal Rabbit IgG | Santa Cruz |
| Vimentin | 1:50 | Monoclonal mouse IgG (RV202) | Santa Cruz |
| E-cad | 1:200 | Polyclonal Rabbit IgG | Santa Cruz |