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Indications of NAM-induced apoptosis in MCF-7 cells (all occurred in a time-dependent manner). (A) Chromatin condensation and apoptotic bodies (as indicated by white arrow), detected by Hoechst 33342 staining. (B) Increased sub-G1 cells population, detected by flow cytometry. (C) Increased Annexin V protein, detected by Annexin V+/PI− staining. NAM, nicotinamide; PI, propidium iodide.
