Figure 1.

(A) RT-PCR analysis of RhoGDI2 and GAPDH mRNA in two human gastric carcinoma patients. In patient 25, RhoGDI2 mRNA was not expressed in the T or N mucosa. However, in patient 26, RhoGDI2 mRNA was expressed in the T but not the N mucosa. (B) Representative IHC staining with RhoGDI2 polyclonal antibody in gastric cancer, showing strong staining for RhoGDI2 in the cytoplasm of the tumor tissue (×400). (C) In the N gastric mucosa, RhoGDI2 was not observed in the epithelial cells (×400). The samples shown in (B) and (C) are derived from patient 26. RT-PCR, reverse transcription-polymerase chain reaction; RhoGDI2, Rho GDP dissociation inhibitor 2; GAPDH, glyceraldehyde-phosphate dehydrogenase; T, tumor; N, normal; IHC, immunohistochemistry.