Fig. 2.

Spatial control over the activation of lipoplexes using immobilized TCEP. (A-F) Phase contrast (A,C,E) and fluorescence (B,D,F) micrographs showing different-sized clusters of TCEP-immobilized beads deposited in circular patterns in a culture dish containing cells and a uniform dispersion of oxidized BFDMA lipoplexes. Beads were allowed to sit for 1 h before removal; cells were then incubated for 48 h before imaging by fluorescence microscopy. (G-H) Phase contrast (G) and fluorescence (H) micrographs showing patterns of EGFP expression in cells created by manual writing with a suspension of TCEP-immobilized beads.