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Zeta Potentials of Lipoplexes Before and After Reduction.[a]
| Lipoplex Sample | Zeta Potential (mV) |
|---|---|
| DNA/BFDMAOX | –25 ± 1 |
| DNA/BFDMAOX + AA | –8 ± 1 |
| DNA/BFDMAOX + TCEP beads | –14 ± 2 |
Lipoplexes were prepared in aqueous Li2SO4 and diluted in culture medium to a BFDMA concentration of 30 μM. Oxidized BFDMA/DNA charge ratios were fixed at 4.2:1. Lipoplexes were then treated with a 10-fold molar excess of AA or TCEP-immobilized polymer beads.
