Figure 4.

miR-92 was identified to affect the proliferative and invasive activity of SiHa cells and the expression of PTEN. (A) A CCK-8 assay was used to detect the proliferation of miR-92 on SiHa cells and the OD was determined every 24 h. (B and C) A Transwell migration assay was used to detect the invasive activity of the transfected SiHa cells (^*P<0.05) The number of cells traversing the slide was determined by averaging five random fields (magnification, ×200) (D) Western blot analysis was used to detect PTEN protein expression when miR-92-mimic and anti-miR-92 were transfected to SiHa cells. miR-92, microRNA-92, PTEN, phosphatase and tensin homologue; CCK, cell counting kit; OD, optical density.