Figure 5.

Effects of miR-92 expression on apoptosis among SiHa cells. (A) Relative caspase-3 activity in control or miR-92 inhibitor-transfected SiHa cells. (B) PCR was performed to detect the expression of PTEN mRNA in SiHa cells transfected with control and anti-miR-92. (C) TUNEL assay was performed to detect apoptosis of SiHa cells transfected with control or anti-miR-92 (arrows, apoptotic nuclei in SiHa cells; magnification, ×400). All experiments were performed in triplicate. miR-92, microRNA-92; PTEN, phosphatase and tensin homologue; TUNEL, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling; NC, negative control.