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. 2013 Jul 1;6(3):840–844. doi: 10.3892/ol.2013.1434

Figure 2.

Figure 2

Efficacy of BBR and COP in sensitizing the cells to TRAIL-induced apoptosis. (A) MDA-MB-231 or (B) MDA-MB-468 cells were pre-incubated with various concentrations of COP for 30 min and further incubated in the presence or absence of TRAIL (50 ng/ml) for 24 h. Absorbance was measured following incubation with WST-1 reagent and cell viability was calculated as a percentage compared with untreated control cells. Data are expressed as the mean ± SEM of at least three triplicate experiments and significant differences between groups are shown as *P<0.05. (C) MDA-MB-468 cells were pretreated with BBR or COP (55 μM) for 90 min, followed by incubation in the presence or absence of TRAIL (50 ng/ml) for 3 h. Whole cell lysates were analyzed by immunoblotting for the indicated proteins. TRAIL, TNF-related apoptosis-inducing ligand; BBR, berberine; COP, coptisine; pp53, phosphorylated p53; pp65, phosphorylated p65.