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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Electrophoresis. 2013 Jul 15;34(15):2268–2274. doi: 10.1002/elps.201300036

Figure 2.

Figure 2

Electropherograms of validation tests in positive and negative controls. (1) positive control #1-- a complex with 3530 uM 8-OHdG standard; (2) Positive control #2 -- a complex with 35 uM 8-OHdG standard; (3) Negative control #1-- a complex with primary and secondary antibody; (4) Negative control #2 -- secondary antibody alone; (5) Negative control #3 -- primary antibody alone. Hydrodynamic injection at pH 9.5 and 3.4 kPa for 5 s; separation in 20 mM sodium tetraborate buffer at 17 kV. Arrowhead: secondary antibody; Arrow: 8-OHdG. Top trace is offset in the y-axis for the clarity.