Figure 1. FCS measurements of Cy5 and the purified LHCII in solution.

Typical stationary time-courses of fluorescence fluctuations emitted from Cy5 (a) and the purified LHCII (b), both in solution, were shown. Both were measured in a coverglass chamber. (c, d) From the obtained FAFs (dots), the mean diffusion coefficients (D) of both samples were calculated by fitting the FAFs with the one-component model (lines) (see equation 2 in Methods). To obtain the diffusion coefficient of the purified LHCII, the published diffusion coefficient for Cy5 (280 μm² s⁻¹) was used for the standard²²,²³.