Figure 2.
(a) Human MM cell lines demonstrate differential expression of Bcl-2 prosurvival proteins. JJN3, OPM-2, RPMI-8226 and U266 were assessed for the expression of antiapoptotic Bcl-2 proteins by western blot: Bcl-2, Bcl-XL, Bcl-W, Mcl-1 and A1. (b) Differential sensitivities of human MM cell lines to ABT-737. Single-agent dose–response curves were constructed in human MM cell lines (JJN3, OPM-2, RPMI-8226 and U266) treated with ABT-737 for 24 and 48 h. (c) Synergistic induction of apoptosis in human MM cell lines JJN3, OPM-2, RPMI-8226 and U266 following 48 h treatment with panobinostat in combination with ABT-737 after 48 h incubation. *P<0.05 versus single agents. Calcusyn was used to determine synergy when the two agents were combined: synergy is determined when CI<0.9, additively when CI is between 0.9 and 1.1 and antagonism when CI>1.1. CI values are shown on each graph