Figure 3.

Differential expression of p21 and 14-3-3σ affects sensitivity to chemotherapeutics. (a) Indicated cell lines were exposed to gemcitabine (10 μM), Cr(VI) (30 μM) or γ-IR (10 Gy) for 48 h. Immunoblotting was carried out for p21, 14-3-3σ and β-actin. (b) Cells were treated as indicated and qRT-PCR was carried out 24 h post damage for p21, 14-3-3σ and GAPDH. Each assay was conducted in quadruplicate and each set of experiments was repeated three times. (c) The viability of cell lines with p21 or 14-3-3σ knocked out or knocked down was measured after 72 h exposure to the indicated DNA-damaging agents. Results are mean±S.D.; ***P<0.0001. Each assay was conducted in triplicate, N=3