Figure 1.

Simulated ischemia increases the levels of multiple inflammasome proteins in primary cortical neurons. (a and b) Representative immunoblots and quantification of inflammasome proteins, and IL-1β and IL-18, in lysates of cortical neurons at the indicated time points during GD. (c and d) Representative immunoblots and quantification of inflammasome proteins, IL-1β and IL-18 in lysates of cortical neurons at the indicated time points during OGD. (e and f) Representative immunoblots and quantification of inflammasome proteins, IL-1β and IL-18 in lysates of cortical neurons after simulated I/R. β-actin was used as a loading control. Data are represented as mean±S.E.M. n=5 cultures. ***P<0.001 compared with control