Figure 4.

Inhibition of caspase-1 reduces inflammasome activation and cell death in primary cortical neurons subjected to ischemia-like conditions. (a and b) Representative immunoblots and quantification illustrating the effect of increasing concentrations (μM) of Ac-YVAD.CMK on levels of cleaved caspase-1 and caspase-3 proteins in primary cortical neurons subjected to GD. (c) The effect of Ac-YVAD.CMK treatment on cell death (%) in primary cortical neurons subjected to GD. (d and e) Representative immunoblots and quantification illustrating the effect of 30 μM and 100 μM Ac-YVAD.CMK treatment on inflammasome proteins, IL-1β, IL-18, and cleaved caspase-3 in primary cortical neurons subjected to OGD. β-actin was used as a loading control. Data are represented as mean±S.E.M. n=5–6 cultures. ***P<0.001 compared with control