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. 2013 Sep 5;4(9):e793. doi: 10.1038/cddis.2013.329

Figure 1.

Figure 1

Treatment of porcine pancreatic elastase (PPE) increased the expression and secretion of placenta growth factor (PlGF) in lung epithelial (LE) cells. Mouse alveolar type II epithelial cells, MLE-15, were washed twice with PBS then incubated in the medium without serum and treated with 0–60 mU/ml PPE for 24 h (a–d), 4 h (e) or 1 h (f). The level of PlGF in the culture medium was detected by ELISA (a). The activity of PlGF promoter in the cellular lysate was evaluated by luciferase activity (b). The mRNA level of PlGF was determined by reverse transcriptional PCR with primer sets for PlGF and GAPDH cDNA (c). Cellular lysates were also subjected to western blot analysis with antibodies for PlGF (d) or Egr-1 (e) and β-actin. The association of Egr-1 and PlGF promoter was evaluated by chromatin immunoprecipitation assay (f). Data are presented as mean±S.E.M. *P<0.05 versus vehicle-treated group