Figure 5.

Recurrent Ca²⁺ flashes are variable, rapid, asynchronous, and prevalent. Photoreceptors from homozygous double-transgenic Tg(Trß2:tdTomato;TαCP:GCaMP3) pde6c^w59 fish were rapidly imaged every 5 s for 15–30 min. Only the GCaMP3 channel is shown. (a) Montage of a representative cell (cell 13; see b and c) at select time points show rapid transient increases in GCaMP3 fluorescence occurring in both the cell body and synapse. Time=(min:s). (b) Representative image (time) projection from a straightened Tg(Trß2:tdTomato;TαCP:GCaMP3) pde6c^w59 retina shows GCaMP3 fluorescence in all cones. The shaded areas represent the areas used to generate the kymograph (c) for the cell body (magenta) and for the synapse (syn; green). All identifiable cells are numbered below and correspond to a column on the kymograph in c. (c) Representative kymograph of GCaMP3 signal across all the time points for both the cell body (magenta) and synapse (green). The boxed areas on both the kymograph (c) and the straightened retina (b) represent the cell (cell 13) and time period shown in the montage in a