Figure 4.

The coordination of PIAS1 and Smad4 in zinc-induced LNCaP cell apoptosis and Smad4 nuclear translocation. (a) PIAS1-coordinated Smad4 promotes zinc-induced cell apoptosis. LNCaP cells transfected with indicated plasmids were treated with 100 μM zinc for 24 h. The apoptotic percentages of cells were measured by flow cytometry. Columns, mean (n=3); bars, S.D. ***P<0.001 versus zinc-treated control group (one-way ANOVA). (b) PIAS1 promotes Smad4 translocation into nucleus in zinc-treated cells. LNCaP cells transiently cotransfected with empty vector, Smad4 alone or with PIAS1 for 24 h were exposed to 100 μM zinc. Cells were fixed and stained with anti-Flag (red) and anti-Myc (green) antibodies