Skip to main content
. 2013 Sep 6;3(9):e144. doi: 10.1038/bcj.2013.42

Figure 2.

Figure 2

Inhibition of PKR activity leads to decreased PP2A activity and increased phosphorylation of Bcl-2. (a) Western blot to evaluate protein levels of PKR, total Bcl-2, phosphor Bcl-2 (S70) in REH Sicontrol vs SiPKR cells. (b) PP2A activity in Sicontrol and SiPKR cells was measured by in vitro assay. (c) REH cells were pretreated with various concentrations of PKR inhibitor for 12 h before being lysed and subjected to western blot to evaluate protein levels of total PKR, phosphor PKR (T451), total EIF2α, phosphor EIF2α (S51), total Bcl-2 and phosphor Bcl-2 (S70). (d) PP2A activity assay was performed to evaluate PP2A activity in PKRI-treated cells. PP2A activity is presented as percentage of control, and representative results from three independent experiments are shown.