Figure 4.

Loss of PKR stabilizes Bcl-2/Bax binding and inhibits Bax insertion to OMM. (a) REH or (b) K562 cells were exposed to H₂O₂ for 6 h, lysed and co-immunoprecipitation of Bcl-2 and Bax performed. (c) REH cells were treated with H₂O₂ for 6 h. Mitochondria were isolated and treated with alkaline buffer. Mitochondrial debris was then collected by ultracentrifugation and subject to western blot for evaluating BAX level. (d) K562 cells were treated with H₂O₂ for 6 h. Bax insertion was evaluated as described previously. Total cellular levels of Bax and Bcl-2 after 6-h exposure to H₂O₂ were evaluated by western blot.