Figure 4.

Discordant patterns of induced antibody response in identically vaccinated patients. (a) Color code for the heatmap of normalized intensity (∼titer) from dark blue (minimum/threshold of detection) to bright red (maximal, equal or greater to level seen for positive control). Antigens unrecognized at timepoint are coded in gray, missing samples are coded in white. (b) Normal donor reactivity against candidate LAAs. Threshold for detection was set at 85% of normal donor distribution to limit false positive results in patient dataset. As predicted by this, experimentally observed normal donor IgG reactivity to these 14 discovered LAAs was rare, and when responses were present they were low titer. (c) Normalized intensity heatmap of CML patient LAA-specific IgG responses before and after both primary and secondary vaccination. All patients received identical K562/GVAX whole-cell vaccine product at identical time points but exhibited markedly divergent responses.