Fig. 6.

RTL binding to monocytes blocks MIF signaling and induces random migration. a) Splenocytes were isolated from 3 naïve DR*1501-Tg mice and treated with 10 μg/ml RTL342M (pDR2/mMOG-35-55) or buffer for 1 h, followed by 10 ng/ml LPS stimulation with or without 100 ng/ml MIF for 1 h and mRNA isolated. Relative expression of ICAM-1 was measured by real-time PCR (*p < 0.05). b–d) Isolated GFP⁺CD11b⁺ cells from DR*1501/GFP-Tg mice were treated with 50 μg/ml RTL342M for 2 h. Ten time-lapse fields (5 untreated and 5 RTL342M treated fields) were imaged by live fluorescence microscopy. b) Mean cell speed (micrometers per second) (***p < 0.0005). c) Mean track displacement length (square micrometers) (***p < 0.0005) of 10 fields. d) Tracks of individual cells (9–11 cells in each field) from four representative fields. Differences analyzed by Student’s T-test.