Fig. 2. Mutation of the zinc coordinating cysteine residues to alanine changes the nuclear localization of 2xEGFP-16E7.
HeLa cells were transfected with either 2xEGFP-16E7 wild type (panels A-C), 2xEGFP-16E7CC58AA (panels D-F), 2xEGFP-16E7C91A (panels G-I), or 2xEGFP (panels J-L) plasmids and examined by confocal fluorescence microscopy at 24 h post transfection. Panels A, D, G and J represent the DAPI staining of the nuclei, panels B, E, H and K represent the fluorescence of the EGFP and panels C, F, I, and L represent the merge.