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. Author manuscript; available in PMC: 2014 Nov 1.

Published in final edited form as: Virology. 2013 Sep 10;446(0):334–345. doi: 10.1016/j.virol.2013.08.017

Fig. 3 — A. Single cysteine substitution to alanine within the zinc finger alters the nuclear localization of 2xEGFP-16E7. HeLa cells were transfected with either 2xEGFP-16E7 wild type (panels A-C), 2xEGFP-16E7_C58A (panels D-F), 2xEGFP-16E7_C59A (panels G-I), or 2xEGFP (panels J-L) plasmids and examined by confocal fluorescence microscopy at 24 h post-transfection. Panels A, D, G and J show the DAPI staining of the nuclei, panels B, E, H and K represent the fluorescence of the EGFP and panels C, F, I, and L represent the merge. B. Quantitative analysis of the effect of single cysteine substitution within the zinc finger on the localization of EGFP-16E7 and 2xEGFP-16E7. Data from five experiments using EGFP-16E7, EGFP-16E7_C58A, EGFP-16E7_C59A, EGFP, 2xEGFP-16E7, 2xEGFP-16E7_C58A, 2xEGFP-16E7_C59A, and 2xEGFP plasmids were used for quantitative analysis and graphic representation of the localization distribution of transfected HeLa cells. Black bars represent cells exhibiting predominant nuclear localization; gray bars represent cells exhibiting pancellular localization; white bars represent cells exhibiting predominant cytoplasmic localization.

Fig. 3 — A. Single cysteine substitution to alanine within the zinc finger alters the nuclear localization of 2xEGFP-16E7. HeLa cells were transfected with either 2xEGFP-16E7 wild type (panels A-C), 2xEGFP-16E7_C58A (panels D-F), 2xEGFP-16E7_C59A (panels G-I), or 2xEGFP (panels J-L) plasmids and examined by confocal fluorescence microscopy at 24 h post-transfection. Panels A, D, G and J show the DAPI staining of the nuclei, panels B, E, H and K represent the fluorescence of the EGFP and panels C, F, I, and L represent the merge. B. Quantitative analysis of the effect of single cysteine substitution within the zinc finger on the localization of EGFP-16E7 and 2xEGFP-16E7. Data from five experiments using EGFP-16E7, EGFP-16E7_C58A, EGFP-16E7_C59A, EGFP, 2xEGFP-16E7, 2xEGFP-16E7_C58A, 2xEGFP-16E7_C59A, and 2xEGFP plasmids were used for quantitative analysis and graphic representation of the localization distribution of transfected HeLa cells. Black bars represent cells exhibiting predominant nuclear localization; gray bars represent cells exhibiting pancellular localization; white bars represent cells exhibiting predominant cytoplasmic localization.