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. 2013 Aug 30;45(8):e38. doi: 10.1038/emm.2013.75

Figure 1.

Figure 1

Phospholipase D (PLD) inhibitors in combination with radiation augment cell growth inhibition in breast cancer cells. (a) MDA-MB-231 cells were treated with different concentrations of the PLD1 or PLD2 inhibitor for 72 h, and cell viability was determined using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. (b) MDA-MB-231 cells were treated with the indicated PLD inhibitor (10 μℳ) for 4 h and were then exposed to 5 Gy radiation. After 72 h, the cell viability was measured using an MTT assay. *P<0.05. (c) The cells were treated with 10 μℳ of the PLD1 or PLD2 inhibitor and/or exposed to 5 Gy ionizing radiation (IR) for 4 h. After 10 days, colonies were fixed with formaldehyde and stained with 2% crystal violet. (d) The number of colonies was counted, and the surviving fraction was normalized to the surviving fraction of the corresponding control. Data are presented as the mean±s.d. of three independent experiments.