Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Sep 18;35(5):317–325. doi: 10.1155/2013/935943

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2013 Li Ma et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

(a) The recombinant vectors pET30a-CCNY-6His were certificated by restricted enzyme cutting. Lane M: DNA marker; Lane 1: recombinant vectors pET30a-CCNY-6His, Lane 2: NCO I single restriction enzyme digestion, Lanes 3: NCO I and Xho I double restriction enzyme digestion. (b) SDS-PAGE analysis was performed to identify the expression of CCNY protein. Lane M: protein molecular weight marker, Lane 1: the negative control of BL21 (DE3), Lane 2: the negative control of BL21 (DE3)/pET30a, Lane 3: CCNY protein expressed in BL21 (DE3)/pET30a. (c) The validation of CCNY autoantibody. Lane 1: positive control (Rabbit anti-CCNY polyclonal antibody), Lane 2: positive control (His-tag antibody), Lanes 3, 4, 5, and 6: the serum with positive CCNY autoantibody, Lanes 7 and 8: the serum with negative CCNY autoantibody, Lane 9: negative control.