Table 2.

Transcriptional levels of cytokines and Toll-like receptors in stimulated splenocytes.

Relative gene expression levelsa in splenocytes from nephritic mice
Genes	Nucleosomes			Nucleosome lmw heparin
	20 hours	4 days	7 days	20 hours	4 days	7 days
IL1β	15.87 ± 5.09	77.78 ± 7.92*	72.69 ± 29.08*	15.54 ± 4.62	86.84 ± 13.09*	80.86 ± 10.80*
IL6	1.80 ± 0.42	6.91 ± 2.46	35.25 ± 8.08*	1.52 ± 0.57	6.57 ± 1.21	37.25 ± 15.09*
IL10	1.29 ± 0.53	1.90 ± 0.25	3.55 ± 0.12*	1.47 ± 0.48	1.79 ± 0.21	4.45 ± 2.16*
IFN-γ	2,35 ± 0.79	4,10 ± 2.62	10,99 ± 0.38*	3,00 ± 1.18	6,53 ± 4.57	11,20 ± 4.03*
TNFα	4.75 ± 0.32*	5.45 ± 0.82*	1.97 ± 0.34*	5.44 ± 1.23*	8.37 ± 1.24*	2.04 ± 0.14*
CXCL1	110.70 ± 47.16*	51.80 ± 11.37	81.63 ± 46.31*	91.75 ± 27.24*	45.92 ± 4.40	91.75 ± 23.38*
TLR2	1.81 ± 0.75b	10.94 ± 0.65*	19.70 ± 4.40*	1.69 ± 0.48	10.15 ± 1.39*	19.02 ± 2.08*
TLR7	2.09 ± 0.82*	3.21 ± 0.57*	2.30 ± 0.38*	1.47 ± 0.41	2.04 ± 0.12*	2.23 ± 1.10*
TLR8	0.34 ± 0.12*	1.64 ± 0.19*	3.31 ± 0.09*	0.43 ± 0.23	1.77 ± 0.31*	2.61 ± 0.28*
TLR9	1.11 ± 0.23	2.01 ± 0.05	4.08 ± 0.87*	1.98 ± 1.05	2.76 ± 0.36*	5.10 ± 1.79*

^aData is given as fold change compared to medium stimulated splenocytes at the same time point. *Statistically significant (P < 0.05) change in mRNA levels compared to medium stimulated cells. ^bMean values and SD were calculated from triplicates.