Figure 10. Slo1 protein is present in human spermatozoa.
(A) Human sperm immunostaining with primary polyclonal anti-Slo1 antibodies and Cy3-conjugated secondary antibodies. Left and middle panels show Slo1 staining localized to the principal piece of human sperm flagellum. Left panel: nuclei are stained by DAPI. Right panel: DIC image of the same cells. Scale bar is 5 mm. (B) Representative immunoblot of the mouse brain (positive control) and human spermatozoa from two different donors (donor 1 and donor 2: D1 and D2, respectively).