Figure 5. Human, but not mouse KSper is sensitive to the Slo1 channel blocker charybdotoxin (ChTX).
(A) Representative human IKSper traces under control conditions (black) and in the presence of 1 μM ChTX (red) elicited in response to the given voltage ramp. (B) Mean current densities (CDs) ± SEM calculated at +120 mV. CDs (human) were: 65 ± 10 pA/pF (control), 5 ± 1 pA/pF (ChTX), and 49 ± 5 pA/pF (washout). (C) Representative mouse IKSper traces under control conditions (black) and in the presence of 1 μM ChTX (red) elicited in response to the voltage ramp as shown in (A). (D) CDs (mouse) were: 205 ± 8 pA/pF (control) vs 196 ± 8 pA/pF (ChTX). (n), number of experiments. Three human and three mouse sperm cells were used.